Which of these is incorrectly matched?
Correct answer D. DNA ligase-Mapping human chromosome
Topic / 40 questions
Biotechnology MCQs
Biotechnology MCQs are multiple-choice questions on using living organisms and their molecules to make useful products, covering recombinant DNA technology, cloning vectors, DNA ligase, DNA sequencing, tissue culture, transgenic organisms and gene therapy. They help MDCAT and medical entry candidates master this chapter. Each question has an instant answer for quick, reliable self-checking.
Which of these would you not expect to be a biotechnology product?
Correct answer D. Steroid hormones
Which of these is a true statement?
Correct answer A. Both plasmids and viruses can serve as vectors.
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Salt tolerant plants are
Correct answer D. Arabidopsis
A device that bombards a callus with DNA coated microscopic metal particles is
Correct answer C. Particle gun
Cell suspension cultures of
Correct answer C. Both a and b
Coconut milk contains the plant hormone
Correct answer B. Cytokinins
Plants are totipotent because
Correct answer B. A single cell could become a complete plant
Tissue culture does not involve
Correct answer D. Use of generative nucleus in pollen culture
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Which of the following is correct about SCID?
Correct answer B. It is an immune disorder
Transgenic bacteria are produced in large vats called
Correct answer C. Bioreactor
Substance used to terminate DNA at different points in Sanger’s method is
Correct answer D. Dideoxyribonucleoside triphosphate
Full set of genes of an individual is called
Correct answer D. Genome
Plasmid do not carry gene for
Correct answer C. Growth
In cystic fibrosis patient lacks a gene for the transmembrane carrier of the
Correct answer D. Chloride ion
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Two methods for gene therapy are
Correct answer B. Ex-vivo and in-vivo
Cumulus cells are those that
Correct answer D. Cling to the egg after ovulation occurs
For biotechnology products which is the preferable vehicle
Correct answer A. Urine
In vortex method of transgenic animals gene is inserted
Correct answer B. Into egg
An antibody made by soybeans can be used to treat
Correct answer D. Genital berpes
About Biotechnology MCQs
This topic from the MCAT Biology section covers both techniques and applications. Questions ask about the enzyme that seals foreign DNA into a vector, chemical methods of DNA sequencing, fermenters where transgenic bacteria are grown, and what tissue culture does and does not involve. Applications include salt-tolerant and plastic-producing transgenic plants, transgenic animals, DNA analysis for identification and diagnosis, and the gene defect in cystic fibrosis.
Biotechnology is a high-yield chapter in medical entry tests because it connects genetics, molecular biology and real-world medicine. Many questions are factual, so a clear list of enzymes, vectors and examples gives quick marks. Practising these MCQs prepares you for MDCAT in Pakistan, NEET UG in India and the MBBS admission test in Bangladesh, and builds a base for later study in medicine.
Key facts to remember
- Restriction enzymes cut DNA at specific recognition sequences.
- DNA ligase joins, or seals, foreign DNA into a vector such as a plasmid.
- Plasmids are small circular DNA molecules commonly used as cloning vectors in bacteria.
- Cystic fibrosis results from a faulty CFTR gene affecting chloride ion transport.
- Human insulin is produced commercially using genetically engineered bacteria.
- The Maxam-Gilbert method sequences DNA by chemically cleaving it at specific bases.
Exams that include Biotechnology MCQs
Pakistan
- MDCAT
- ETEA
- NTS
India
- NEET UG
- CUET
- ICAR AIEEA
Bangladesh
- Medical admission test (MBBS)
- University admission tests (DU, RU, JU)
How to prepare for Biotechnology MCQs
- List the tools of genetic engineering, restriction enzymes, ligase, vectors and host cells, with the job of each.
- Learn the steps of recombinant DNA technology in order, from isolating the gene to cloning it in a host.
- Make a table of transgenic examples, such as plants and animals, with the trait each one carries.
- Revise genetic disorders linked to gene therapy, such as cystic fibrosis, with the protein or gene involved.
Frequently asked questions
Which enzyme seals foreign DNA into a vector?
DNA ligase seals foreign DNA into a vector. After a restriction enzyme cuts both the vector and the foreign DNA, their matching ends pair up, and DNA ligase forms phosphodiester bonds to join the backbone, producing a stable recombinant DNA molecule.
How many Biotechnology MCQs are on MCQs360?
There are 51 Biotechnology MCQs on MCQs360 in the MCAT Biology section. Each question gives an instant answer, and you can practise the full set free without signing up. It makes a focused revision set for this chapter.
What is a transgenic organism?
A transgenic organism carries a gene from another species introduced by genetic engineering. Examples include bacteria that produce human insulin, crop plants engineered for pest or salt tolerance, and animals modified to produce useful proteins. The new gene is passed on to offspring.
How should I prepare Biotechnology for MDCAT?
Read the textbook chapter carefully, then list enzymes, vectors, techniques and applications with one-line definitions. Practise MCQs to see how facts are tested, and revise mismatched pairs often, since examiners like incorrectly matched questions in this chapter.
Last reviewed October 2026